Table 3

Matched sample frequency cells for the agreement and discordance between the standard of care (ZN smear) and FM, CY and PiLED smears on the culture positive and negative specimens

			FM		CY		PiLED
Culture positive			P	N	P	N	P	N
A: n=72	ZN	P	35	1	34	2	35	1
A: n=72		N	9	27	05	31	08	28
B: n=81	ZN	P	29	0	29	0	29	0
B: n=81		N	7	45	4	48	10	42
C: n=68	ZN	P	29	2	31	0	29	2
C: n=68		N	6	31	2	35	10	27
Culture negative
A: n=78	ZN	P	6	2	7	2	9	0
A: n=78		N	7	63	4	65	12	57
B: n=69	ZN	P	11	1	6	2	14	0
B: n=69		N	10	47	4	57	10	45
C: n=82	ZN	P	11	1	10	1	13	0
C: n=82		N	12	58	6	65	10	59

A, cultured samples decontaminated for 10 min; B, cultured samples decontaminated for 15 min; C, cultured samples decontaminated for 20 min.
CY, CysCope fluorescence microscopy; FM, mercury vapour fluorescence microscopy; P, positive; PiLED, Primo Star iLED fluorescence microscopy; N, negative; ZN, Ziehl Neelsen microscopy.