Stool assays, methods, instruments and processing laboratories
| Stool analyte | Amount of stool (mg) | Assay method | Instrument | Processing lab |
| Calprotectin | 100 | Sandwich Immunoassay | Liaison immune analyzer, Diasorin. | NRL, AKU, Pakistan |
| Lipocalin-2 | 100 | Sandwich Immunoassay | Epoch 2 microplate reader, Biotek. | NRL, AKU, Pakistan |
| Claudin-15 | 300 | Competitive Enzyme Immunoassay | Epoch 2 microplate reader, Biotek. | NRL, AKU, Pakistan |
| RNA extraction | 250 | Column Extraction Technology | ZymoBIOMICS RNA Miniprep Kit (R2001) | NRL, AKU, Pakistan |
| DNA extraction | 200 | Column Extraction Technology | ZymoBIOMICS DNA Miniprep Kit (D4300) | NRL, AKU, Pakistan |
| Stool archiving for shipment | 2000 | – | ThermoScientific TSX-Series −80°C Ultralow temperature freezer | NRL, AKU, Pakistan |
| Stool metabolomics* | 100 | Microbiome Metabolism Assay | LC-MS | TMIC, Alberta, Canada |
*short-chain fatty acids (SCFAs), amino acids, intermediates in glycolysis and nucleotide metabolism.
AKU, Aga Khan University’s; LC-MS, Liquid chromatography mass spectrometry; NRL, Nutrition Research Laboratory; TMIC, The Metabolomics Innovation Centre.