Pruritus-specific predictors and outcome measures
| Predisposing, triggering and maintaining/aggravating factors | Single constructs | Instrument | Months | ||
| 0 | 6 | 12 | |||
| Cellular metabolism | Multiphoton tomographic fluorescence lifetime imaging (MPT-FLIM) Hyperspectral imaging (HSI) | X X | X X | X X | |
| Skin barrier nanostructure | Atomic force microscopy (AFM) Trans-epidermal water loss measurement (TEWL) | X X | X X | X X | |
| Cutaneous nerve fibre anatomy | Biopsies, immunohistochemistry, blood samples | X | X | ||
| Molecular markers | qPCR (NGF, semaphorin 3A, artemin) | X | X | ||
| Indicators of central sensitisation | Quantitative sensory testing (QST) Alloknesis | X X | X X | X X | |
| Proinflammatory marker | Blood: ELISA for relevant marker IL31 | X | X | ||
| Pruritus-specific outcome variables (assessed via self-report) | |||||
| Primary | Pruritus intensity | NRS: worst pruritus in the last 24 hours | X | X | X |
| Secondary | Pruritus-related impairment and life quality | Pruritus intensity scalesItchyQol, itch controlled days, scratch pleasure rating (NRS) | X | X | X |
NRS, numeric rating scale.