Primer and probe sets used for RT-qPCR ZIKV detection
| Primer/probe set | Primer and/or probe | Genome position | Sequence (5’ – 3’) |
| Set 1 | ZIKV 835 | 835–857 | TTGGTCATGATACTGCTGATTGC |
| ZIKV 860-FAM | 860–886 | CGGCATACAGCATCAGGTGCATAGGAG | |
| ZIKV 911 c | 911–890 | CCTTCCACAAAGTCCCTATTGC | |
| Set 2 | ZIKV 1086 | 1086–1102 | CCGCTGCCCAACACAAG |
| ZIKV 1107-FAM | 1162–1139 | AGCCTACCTTGACAAGCAGTCAGACACTCAA | |
| ZIKV 1162 c | 1107–1137 | CCACTAACGTTCTTTTGCAGACAT |
Primers designed by Lanciotti et al, 2008 (Based on ZIKV MR 766 GenBank accession no. AY632535).
FAM, fluorescent 6-carboxyfluorescein; PCR, polymerase chain reaction; RT-qPCR, real time quantitative PCR; ZIKV, Zika virus.