Analytical methods used to measure biochemical analytes on the Olympus AU5400 Automated Chemistry analyser
| Analyte | Method | Bias (%) | CV (%) |
| TG | GPO-PAP | 0.78 | 1.95 |
| TC | CHOD-PAP | −0.66 | 1.87 |
| HDL-C | Direct clearance | −4.76 | 2.51 |
| LDL-C | Direct clearance | 4.44 | 2.21 |
| ApoA1 | Immunoturbidimetric | 0.06 | 1.53 |
| ApoB | Immunoturbidimetric | −2.98 | 2.14 |
The total analytical imprecision for the experimental method used to calculate the reference intervals is given for each test as an average coefficient of variation (CV%) of two levels of internal controls through 1 year.
ApoA1, apolipoprotein A1; ApoB, apolipoprotein B; CHOD-PAP, cholesterol oxidase-peroxidase-phenol-4-aminoantipyrine; CV, coefficient of variation; GPO-PAP, glycerol-3-phosphate oxidase-peroxidase; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; TC, total cholesterol; TG, triglycerides.