Table 1

Summary of the PREGRALL study

	Prenatal			Postnatal
Weeks’ gestation	20*	24†	32†
Age of the infant				Day 0		Day 1–5		Month 1†	Month 2†		Month 3†	Month 6†	1 year†
Participant	Mother		Mother	Mother	Infant	Mother	Infant		Mother	Infant			Mother	Infant
Informed consent taken and study information provided	x
Study treatment supplied	x		x
Samples‡
Blood (53 mL mother, 8 mL infant)	x		x	x					x					x
Stool (5 g)	x§		x§	x			x		x§	x§				x
Buccal swabs (in 1 mL RNAlater)	x		x	x			x		x	x			x	x
Cord blood (20 mL)					x
Placental biopsy					x
Transepidermal water loss							x							x
Colostrum (2 mL)/milk (10 mL)						x		x§¶
Assessments
ISAAC questionnaire												x¶
SCORAD score														x
FDLQI score													x	x
Prebiotic tolerance		x¶	x	x
Skin prick testing for common allergens														x
Documents
National Guidelines for Child Dietary Diversification											x**

*± 6 days.
†± 1 week.
‡Only undertaken by participants included in the Characterising the effect of maternal prebiotic supplementation on perinatal Immune system maturation, Microbiota and breast Milk compositions for Allergy Prevention in high-risk children study (quantities listed are per collection).
§Sample collection undertaken home.
¶Telephone call.
**Sent by post or email.
FDLQI, Family Dermatitis Quality of Life Index; ISAAC, International Study of Asthma and Allergies in Childhood; SCORAD, Scoring Atopic Dermatitis.