Echocardiographic imaging protocol required views
| Parasternal position | |
| Parasternal long axis | Two-dimensional (2D) imaging (at deep depth) 2D imaging (at shallow depth) Colour Doppler of the mitral and aortic valves (AVs) |
| Parasternal short axis, AV level | 2D imaging of AV Colour Doppler of AV 2D imaging of right ventricular outflow tract (RVOT) Colour Doppler of RVOT Pulsed-wave (PW) and Continous-wave (CW) Doppler of RVOT |
| Parasternal short axis, mitral valve level | 2D imaging |
| Parasternal short axis, left ventricle apex | 2D imaging |
| Apical position | |
| Apical four-chamber view | 2D imaging 2D imaging, focused/zoomed of left ventricle (LV) 2D imaging, focused on left atrium Colour Doppler of mitral valve/left atrium PW Doppler of mitral flow CW Doppler of mitral flow Tissue Doppler imaging (TDI) of septal and lateral mitral annulus |
| Apical four-chamber view, focused on the right ventricular | 2D imaging Colour Doppler of tricuspid valve/right atrium CW Doppler of tricuspid regurgitation TDI of lateral tricuspid annulus |
| Apical five-chamber view | 2D imaging Colour Doppler of left ventricular outflow tract (LVOT) PW of LVOT flow CW of transaortic flow |
| Apical two-chamber view | 2D imaging 2D imaging focused/zoomed on LV 2D imaging focused on left atrium Colour Doppler mitral valve/left atrium |
| Apical three-chamber view | 2D imaging 2D imaging focused/zoomed on LV 2D imaging focused on left atrium Colour Doppler mitral valve/left atrium Colour Doppler of AV PW of LVOT flow CW of transaortic flow |
| Subcostal view | |
| Inferior vena cava | 2D imaging (5 s acquisition) |